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sisti  

Real-Time PCR Data Sets by Sisti et al. (2010)
View on CRAN: Click here


Download and install sisti package within the R console
Install from CRAN:
install.packages("sisti")

Install from Github:
library("remotes")
install_github("cran/sisti")

Install by package version:
library("remotes")
install_version("sisti", "0.0.1")



Attach the package and use:
library("sisti")
Maintained by
Ramiro Magno
[Scholar Profile | Author Map]
All associated links for this package
First Published: 2024-04-28
Latest Update: 2024-04-28
Description:
This data package contains four datasets of quantitative PCR (qPCR) amplification curves that were used as supplementary data in the research article by Sisti et al. (2010), <doi:10.1186/1471-2105-11-186>. The primary dataset comprises a ten-fold dilution series spanning copy numbers from 3.14 × 10^7 to 3.14 × 10^2, with twelve replicates per concentration. These samples are based on a pGEM-T Promega plasmid containing a 104 bp fragment of the mitochondrial gene NADH dehydrogenase 1 (MT-ND1), amplified using the ND1/ND2 primer pair. The remaining three datasets contain qPCR results in the presence of specific PCR inhibitors: tannic acid, immunoglobulin G (IgG), and quercetin, respectively, to assess their effects on the amplification process. These datasets are useful for researchers interested in PCR kinetics. The original raw data file is available as Additional File 1: <https://static-content.springer.com/esm/art%3A10.1186%2F1471-2105-11-186/MediaObjects/12859_2009_3643_MOESM1_ESM.XLS>.
How to cite:
Ramiro Magno (2024). sisti: Real-Time PCR Data Sets by Sisti et al. (2010). R package version 0.0.1, https://cran.r-project.org/web/packages/sisti. Accessed 23 Nov. 2024.
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