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net4pg
View on CRAN: Click
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Download and install net4pg package within the R console
Install from CRAN:
install.packages("net4pg")
Install from Github:
library("remotes")
install_github("cran/net4pg")
Install by package version:
library("remotes")
install_version("net4pg", "0.1.1")
Attach the package and use:
library("net4pg")
Maintained by
Laura Fancello
[Scholar Profile | Author Map]
[Scholar Profile | Author Map]
All associated links for this package
10.32614/CRAN.package.net4pg . https://github.com/laurafancello/net4pg/issues . https://github.com/laurafancello/net4pg . net4pg results . net4pg.pdf . An introduction to net4pg . net4pg_0.1.1.tar.gz . net4pg_0.1.1.zip . net4pg_0.1.1.zip . net4pg_0.1.1.zip . net4pg_0.1.1.tgz . net4pg_0.1.1.tgz . net4pg_0.1.1.tgz . net4pg_0.1.1.tgz . net4pg archive . https://CRAN.R-project.org/package=net4pg .
First Published: 2021-09-20
Latest Update: 2022-09-07
Description:
In shotgun proteomics, shared peptides (i.e., peptides that might
originate from different proteins sharing homology, from different
proteoforms due to alternative mRNA splicing, post-translational
modifications, proteolytic cleavages, and/or allelic variants) represent a
major source of ambiguity in protein identifications. The 'net4pg' package
allows to assess and handle ambiguity of protein identifications. It
implements methods for two main applications. First, it allows to represent
and quantify ambiguity of protein identifications by means of graph
connected components (CCs). In graph theory, CCs are defined as the largest
subgraphs in which any two vertices are connected to each other by a path
and not connected to any other of the vertices in the supergraph. Here,
proteins sharing one or more peptides are thus gathered in the same CC
(multi-protein CC), while unambiguous protein identifications constitute CCs
with a single protein vertex (single-protein CCs). Therefore, the proportion
of single-protein CCs and the size of multi-protein CCs can be used to
measure the level of ambiguity of protein identifications. The package
implements a strategy to efficiently calculate graph connected
components on large datasets and allows to visually inspect them.
Secondly, the 'net4pg' package allows to exploit the increasing
availability of matched transcriptomic and proteomic datasets to
reduce ambiguity of protein identifications. More precisely, it
implement a transcriptome-based filtering strategy fundamentally
consisting in the removal of those proteins whose corresponding
transcript is not expressed in the sample-matched transcriptome. The
underlying assumption is that, according to the central dogma of
biology, there can be no proteins without the corresponding
transcript. Most importantly, the package allows to visually inspect
the effect of the filtering on protein identifications and quantify
ambiguity before and after filtering by means of graph connected
components. As such, it constitutes a reproducible and transparent
method to exploit transcriptome information to enhance protein
identifications. All methods implemented in the 'net4pg' package are fully
described in Fancello and Burger (2022) .
How to cite:
Laura Fancello (2021). net4pg: Handle Ambiguity of Protein Identifications from Shotgun Proteomics. R package version 0.1.1, https://cran.r-project.org/web/packages/net4pg. Accessed 30 Apr. 2025.
Previous versions and publish date:
0.1.0 (2021-09-20 17:20)
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Other R packages that net4pg depends,
imports, suggests or enhances
Complete documentation for net4pg
Functions, R codes and Examples using
the net4pg R package
Some associated functions: cc_composition . cc_stats . get_adj_matrix . get_cc . peptide_stats . plot_cc . read_inc_matrix . reduce_inc_matrix . transcriptome_filter .
Some associated R codes: cc_composition.R . cc_stats.R . get_adj_matrix.R . get_cc.R . global_variables.R . peptide_stats.R . plot_cc.R . read_inc_matrix.R . reduce_inc_matrix.R . transcriptome_filter.R . Full net4pg package functions and examples
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