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freqpcr  

Estimates Allele Frequency on qPCR DeltaDeltaCq from Bulk Samples
View on CRAN: Click here


Download and install freqpcr package within the R console
Install from CRAN:
install.packages("freqpcr")

Install from Github:
library("remotes")
install_github("cran/freqpcr")

Install by package version:
library("remotes")
install_version("freqpcr", "0.4.0")



Attach the package and use:
library("freqpcr")
Maintained by
Masaaki Sudo
[Scholar Profile | Author Map]
All associated links for this package
First Published: 2022-01-27
Latest Update: 2022-01-27
Description:
Interval estimation of the population allele frequency from qPCR analysis based on the restriction enzyme digestion (RED)-DeltaDeltaCq method (Osakabe et al. 2017, ), as well as general DeltaDeltaCq analysis. Compatible with the Cq measurement of DNA extracted from multiple individuals at once, so called "group-testing", this model assumes that the quantity of DNA extracted from an individual organism follows a gamma distribution. Therefore, the point estimate is robust regarding the uncertainty of the DNA yield.
How to cite:
Masaaki Sudo (2022). freqpcr: Estimates Allele Frequency on qPCR DeltaDeltaCq from Bulk Samples. R package version 0.4.0, https://cran.r-project.org/web/packages/freqpcr. Accessed 22 Dec. 2024.
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