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debar  

A Post-Clustering Denoiser for COI-5P Barcode Data
View on CRAN: Click here


Download and install debar package within the R console
Install from CRAN:
install.packages("debar")

Install from Github:
library("remotes")
install_github("cran/debar")

Install by package version:
library("remotes")
install_version("debar", "0.1.1")



Attach the package and use:
library("debar")
Maintained by
Cameron M. Nugent
[Scholar Profile | Author Map]
All associated links for this package
First Published: 2019-12-22
Latest Update: 2024-01-12
Description:
The 'debar' sequence processing pipeline is designed for denoising high throughput sequencing data for the animal DNA barcode marker cytochrome c oxidase I (COI). The package is designed to detect and correct insertion and deletion errors within sequencer outputs. This is accomplished through comparison of input sequences against a profile hidden Markov model (PHMM) using the Viterbi algorithm (for algorithm details see Durbin et al. 1998, ISBN: 9780521629713). Inserted base pairs are removed and deleted base pairs are accounted for through the introduction of a placeholder character. Since the PHMM is a probabilistic representation of the COI barcode, corrections are not always perfect. For this reason 'debar' censors base pairs adjacent to reported indel sites, turning them into placeholder characters (default is 7 base pairs in either direction, this feature can be disabled). Testing has shown that this censorship results in the correct sequence length being restored, and erroneous base pairs being masked the vast majority of the time (>95%).
How to cite:
Cameron M. Nugent (2019). debar: A Post-Clustering Denoiser for COI-5P Barcode Data. R package version 0.1.1, https://cran.r-project.org/web/packages/debar. Accessed 07 Mar. 2026.
Previous versions and publish date:
0.1.0 (2019-12-22 16:50)
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